Jacqueline Humphries lives and works in New York. Her work was included in the 59th Venice Biennale, The Milk of Dreams, in 2022, and was the subject of a major survey at the Wexner Center for the Arts, Columbus, in 2021. Other solo shows include the Aspen Art Museum (2025–26); Greene Naftali, New York (2022–23, 2017); Dia Art Foundation, The Dan Flavin Art Institute, Bridgehampton, New York (2019); Carnegie Museum of Art, Pittsburgh (2015); and Contemporary Art Center New Orleans (2015). Significant group shows include those held at the Museum of Contemporary Art, Chicago (2024); National Gallery of Art, Washington, DC (2022); Hirshhorn Museum and Sculpture Garden, Washington, DC (2019); Museum Brandhorst, Munich (2019, 2015); San Francisco Museum of Modern Art (2016); Tate Modern (2015); and the 2014 Whitney Biennial, among others.

Humphries's work is in the collections of The Museum of Modern Art, New York; Whitney Museum of American Art, New York; Solomon R. Guggenheim Museum, New York; Metropolitan Museum of Art, New York; Albright-Knox Art Gallery, Buffalo; The Art Institute of Chicago; Museum of Contemporary Art, Chicago; Museum of Fine Arts, Boston; Hirshhorn Museum and Sculpture Garden, Washington, DC; Museum of Contemporary Art, Los Angeles; Carnegie Museum of Art, Pittsburgh; Glenstone Museum, Potomac, Maryland; Hessel Museum of Art, Annandale-on-Hudson, New York; San Francisco Museum of Modern Art; de Young Museum, San Francisco; Tate Modern, London; Centre Pompidou, Paris; Fondation Louis Vuitton, Paris; Aïshti Foundation, Lebanon; and Museum Brandhorst, Munich, among others.